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Features
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Animal-derived component-free
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Protein-free/Peptide ingredients-free
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Chemically Defined
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DMF Filing in Progress
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Universality: Support the culture of various CHO cell lines (CHO-K1, CHO_ZN, Horizon, CHO-S, CHO DG44, etc.) in fed-batch, intensified fed-batch and perfusion modes
Advantages
Equivalent or superior performance to global brand products with better price and lead time
4 advanced CMPM (Cone Mixing Pin Milling) powder media production lines to meet different customer demand with high inter-batch consistency (CPK*>1.33)
Safe and compliant: chemically defined formulation minimizes the potential of contamination from animal-derived components and meets relevant compliance and regulatory requirements
EU certified ISO13485:2016 QMS and MDSAP (FDA), all data are traceable
Full documentation support available for IND application
Strictly selected the "2 domestic+1 imported" raw material supplier model to to ensure a stable and reliable supply chain
*CPK is a standard index to state the capability of one process. CPK=1.33: the process is capable and meets specification limits. The higher the CPK,the better.
Order Information
Basal Medium
Fed-Batch Cultures
Application | Product Name | Cat. No. | Size | Form | Instructions (PDF) | |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden B600S Basal Medium | EXP0109901 | 10 L | Powder | Download | |
EXP0109902 | 200 L | Powder | ||||
Eden B501S Basal Medium | EXP0106001 | 10 L | Powder | Download | ||
EXP0106002 | 200 L | Powder | ||||
Eden B401S Basal Medium | EXP0105901 | 10 L | Powder | Download | ||
EXP0105902 | 100 L | Powder | ||||
EXP0105903 | 200 L | Powder | ||||
Eden B300S Basal Medium | EXP0107901 | 100 L | Powder | Download | ||
EXP0107902 | 200 L | Powder | ||||
EXP0107903 | 10 L | Powder |
Application | Product Name | Cat. No. | Size | Form | Product Instruction (pdf) | |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden F601aS Feed Medium | EXP0112101 | 20 L | Powder | Download | |
EXP0112102 | 10 L | Powder | ||||
EXP0112103 | 1 L | Powder | ||||
Eden F600aS Feed Medium | EXP0108901 | 20 L | Powder | Download | ||
EXP0108902 | 10 L | Powder | ||||
EXP0108903 | 1 L | Powder | ||||
Eden F500aS Feed Medium | EXP0105001 | 1 L | Powder | Download | ||
EXP0105002 | 20 L | Powder | ||||
Eden F400aS Feed Medium | EXP0104801 | 1 L | Powder | Download | ||
EXP0104802 | 20 L | Powder | ||||
Eden F600bS Feed Medium | EXP0108801 | 10 L | Powder | Download | ||
EXP0108802 | 1 L | Powder | ||||
Eden F200bS Feed Medium | EXP0104601 | 1 L | Powder | Download | ||
EXP0104602 | 10 L | Powder | ||||
EXP0104603 | 20 L | Powder | ||||
EXP0104604 | 50 L | Powder |
Performance
Antibodies
In fed-batch mode, CHO cells cultured in Eden CD CHO media demonstrate higher viable cell density (VCD) and protein production than competitors. On average, the antibody titers of various CHO cell lines cultured in Eden CD CHO media ranged from 2 to 11 g/L.
In the pulse perfusion process, CHO cells cultured in Eden CD CHO media demonstrated superior performance than competitive global brands. When VVD=1.0, volumetric productivity (Vp) can reach up to 2.2 g/L/day, and the cumulative product expression in 14 days can reach 19 g/L, 55% higher than global brand B. When VVD=2.0, Vp can reach up to 3.3g/L/day, and the cumulative product expression in 14 days can reach 25 g/L.
Documents
CHO Cell Medium
FAQ
Q1:How is the mixing time determined during medium preparation? If insoluble particles persist after long-term stirring (over 1 hour), how should they be handled?
Q2:Can the Eden series basal medium be used in combination with feed medium 7a/7b?
Q3:BioEngine offers multiple options for CHO cell culture medium. How do I choose the right one?
Q4:Does BioEngine's Eden series CHO CD medium contain Pluronic F-68 (PF-68)?
Q5:What are the packaging sizes available for the Eden series CHO cell culture medium products?
Q6:What is F12 in DMEM?
Q7:What is perfusion culture?
Perfusion culture is commonly used for the production of high-value biopharmaceuticals, such as monoclonal antibodies, where high cell densities are required to achieve sufficient product yields. In a perfusion culture, cells can be grown at higher densities and for longer periods of time, leading to higher productivity and more efficient use of resources than in traditional batch or fed-batch cultures.
Perfusion culture can be operated in various ways, including tangential flow filtration (TFF), spin filters, and hollow fiber membranes, each with its own advantages and limitations. TFF-based perfusion culture is the most used technique due to its scalability, ease of operation, and ability to maintain consistent cell densities and product quality over time.
Q8:What are the applications of CHO cells?
1. Production of biopharmaceuticals: CHO cells are widely used in the production of biopharmaceuticals, such as monoclonal antibodies, growth factors, and hormones. These proteins can be used for the treatment of a range of diseases, including cancer, autoimmune disorders, and diabetes.
2. Drug discovery: CHO cells are often used in drug discovery research to screen and test potential drug candidates. They are particularly useful for testing drugs that target specific receptors or proteins.
3. Vaccine production: CHO cells can be used to produce vaccines, including those for influenza and human papillomavirus (HPV).
4. Genetic engineering: CHO cells can be genetically engineered to produce specific proteins with desired properties. This can be done by introducing genes that encode for the protein of interest into the cells.
5. Toxicology testing: CHO cells are sometimes used in toxicology testing to evaluate the safety of chemicals or drugs. They can be used to test the toxicity of compounds on cells and to study their effects on cellular pathways.
Overall, CHO cells are a valuable tool in biomedicine, and their applications are constantly expanding as new techniques and technologies emerge.
Q9:What are Vero cell lines used for?
Q10:What are low serum media?
Q11:What is a Marc145 cell?
Q12:How do I prepare cell culture media from powder?
Q13:How can cell transfection be classified?
1. Chemical transfection: This method uses chemical agents to facilitate the uptake of nucleic acids into cells. Lipofection and calcium phosphate transfection are examples of chemical transfection methods.
2. Electroporation: This method uses an electric field to create transient pores in the cell membrane, allowing nucleic acids to enter the cell. Electroporation is commonly used for transfection of difficult-to-transfect cells.
3. Viral transfection: This method involves the use of viral vectors, which are modified viruses that can deliver nucleic acids into cells. Lentiviral and retroviral transfection are examples of viral transfection methods.
4. Physical transfection: This method uses physical forces, such as microinjection or gene gun, to deliver nucleic acids into cells.
5. Biolistic transfection: This method uses high-pressure helium gas to deliver nucleic acids into cells. It is also known as the ""gene gun"" method.
6. Magnetofection: This method involves using magnetic nanoparticles to deliver nucleic acids into cells, which are then targeted to specific regions of the cell by applying a magnetic field.
These methods can also be further classified based on whether they are transient or stable. Transient transfection refers to the temporary expression of the introduced nucleic acids, whereas stable transfection results in the integration of the nucleic acids into the host cell genome, resulting in long-term expression of the introduced genes."
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